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Codon usage calculator

Paste a coding sequence and get the full codon table — every codon's count, share, and RSCU (relative synonymous codon usage) — plus GC content at the first, second and third codon positions.

0 bases · Spaces, line numbers and FASTA headers are removed automatically.

Codon table

Good to know

Reading the RSCU column

RSCU = 1 means a codon is used exactly as often as expected if all synonymous codons were equal. Values well above 1 mark preferred codons in your sequence; comparing this profile between a gene and its expression host is the first step of codon optimisation.

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