Tools
Free bioinformatics tools
Small, fast tools for the everyday sequence jobs — transcription, translation, reverse complement, GC content, primer checks and protein weight. Every tool is free, needs no sign-up, and runs entirely in your browser: your sequences never leave your device.
The toolbox
Fourteen tools, ready to use
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01DNA transcription & translation: open the tool
DNA transcription & translation
Convert DNA to mRNA, translate in all six reading frames and find the longest ORF, with GC content and reverse complement. Free · runs in your browser
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02Reverse complement: open the tool
Reverse complement
Get the complement and reverse complement of a DNA sequence — the opposite strand, written 5′→3′ for primers and cloning. Free · runs in your browser
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03GC content calculator: open the tool
GC content calculator
GC and AT percentage, base counts, approximate melting temperature and molecular weight for any DNA or RNA sequence. Free · runs in your browser
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04DNA ↔ RNA converter: open the tool
DNA ↔ RNA converter
Convert DNA to RNA (T→U) or RNA back to DNA (U→T). Both forms shown, cleaned and ready to use. Free · runs in your browser
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05Primer Tm calculator: open the tool
Primer Tm calculator
Melting temperature, GC content and a 3′ GC-clamp quality check for PCR primers. Free · runs in your browser
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06Protein molecular weight calculator: open the tool
Protein molecular weight calculator
Molecular weight in Da and kDa, amino-acid composition and GRAVY score from a protein sequence. Free · runs in your browser
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07Pairwise sequence alignment: open the tool
Pairwise sequence alignment
Global (Needleman–Wunsch) and local (Smith–Waterman) alignment of two sequences, with score, identity and gaps. Free · runs in your browser
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08Restriction digest simulator: open the tool
Restriction digest simulator
Cut positions and fragment sizes for 22 common restriction enzymes, for linear DNA or circular plasmids. Free · runs in your browser
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09ORF finder: open the tool
ORF finder
Find open reading frames in all six reading frames, with strand, coordinates, length and protein sequence. Free · runs in your browser
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10In-silico PCR: open the tool
In-silico PCR
Predict PCR product size and sequence from a primer pair and template before you order primers. Free · runs in your browser
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11Primer pair designer: open the tool
Primer pair designer
Design ranked PCR primer pairs from a template, checked for GC, Tm, product size and 3′ clamp. Free · runs in your browser
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12Codon usage calculator: open the tool
Codon usage calculator
Codon counts, frequency, RSCU and GC at codon positions 1, 2 and 3 for any coding sequence. Free · runs in your browser
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13Protein pI calculator: open the tool
Protein pI calculator
Estimate a protein's isoelectric point and net charge at pH 7 from its ionisable residues. Free · runs in your browser
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14FASTA analyser: open the tool
FASTA analyser
Batch statistics for multi-FASTA files: per-sequence length, GC% and type, plus N50 — with CSV download. Free · runs in your browser
We build new tools regularly. Tell us what you need — or learn to build your own in our bioinformatics training.