Free tool · All tools
In-silico PCR
Check a primer pair against your template before you spend money on synthesis: the tool finds exact binding sites on both strands and reports every product with its size and sequence.
0 bases
Predicted products
Product sequences
Good to know
Exact matches only — by design
This checker uses exact primer matches, so a product here means the pair definitely binds. Real PCR tolerates some mismatches, especially away from the 3′ end — a pair that fails here may still work, but a pair that passes will not surprise you with the wrong product.