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Restriction digest simulator
Paste a DNA sequence, pick your enzymes, and see exactly where each one cuts and the fragment sizes a combined digest would produce on a gel — for linear DNA or a circular plasmid.
0 bases · Spaces, line numbers and FASTA headers are removed automatically.
Enzymes:
Cut sites by enzyme
Fragments
Good to know
Check the ends before you clone
Fragment sizes assume complete digestion at every site. For cloning, also check that your two enzymes leave compatible ends and do not cut inside your insert — the per-enzyme table above makes that a ten-second check.